Murein-hydrolyzing activity of flagellin FlaA of Listeria monocytogenes.
نویسندگان
چکیده
In this preliminary report we show that a 29 kDa surface protein of Listeria monocytogenes EGD removed from cells with 4 M LiCl has peptidoglycan (murein) hydrolyzing activity, as revealed by zymographic analysis using Bacillus subtilis murein and heat-killed Micrococcus luteus cells casted in the gel. Following two-dimensional electrophoresis, the protein was electroblotted to PVDF membrane and its identity (FlaA) was revealed by sequencing. Peptidoglycan hydrolysing activity of FlaA purified by FPLC on Mono-S Sepharose against labelled Escherichia coli murein was demonstrated.
منابع مشابه
Listeria monocytogenes regulates flagellar motility gene expression through MogR, a transcriptional repressor required for virulence.
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ورودعنوان ژورنال:
- Polish journal of microbiology
دوره 53 4 شماره
صفحات -
تاریخ انتشار 2004